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Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: Dec 21, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Fast sequence-based microsatellite genotyping development workflow.

Olivier Lepais^1,2, Emilie Chancerel¹, Christophe Boury¹

¹INRAE, Univ. Bordeaux, BIOGECO, Cestas, France.

|May 16, 2020

Summary

Streamlined microsatellite sequencing (SSRseq) workflows improve population genetic diversity analysis. De novo primer design yields more reliable genetic markers than repurposing old assays for broader species applications.

Keywords:

HapSTR Haplotype sequence SNPSTR SSR-GBS SSR-seq Sequence-based microsatellite genotyping

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

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Last Updated: Dec 21, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

Genomics
Population Genetics
Molecular Biology

Background:

Electrophoresis-based microsatellite genotyping has limitations.
High-throughput sequencing offers improved resolution for genetic diversity and population structure studies.

Purpose of the Study:

To present a streamlined workflow for microsatellite sequencing (SSRseq).
To demonstrate the workflow's effectiveness across diverse species with varying genomic resources.
To identify critical factors for successful SSRseq implementation.

Main Methods:

Microsatellite development and multiplexed marker amplification.
High-throughput sequencing and automated bioinformatics analysis.
Application across fungi, plants, insects, and fish.

Main Results:

De novo primer design resulted in highly multiplexed assays yielding 20-40 reliable loci.
Relying on previously developed assays led to a low number of genotyped loci.
Sequence analysis enabled powerful multi-allelic haplotype-based genotypic datasets.

Conclusions:

Streamlined SSRseq workflows, particularly with de novo primer design, enhance genetic marker reliability and throughput.
Effective SSRseq setup requires careful consideration of upfront development factors.
New analytical frameworks are needed to leverage multi-nucleotide polymorphism data from SSRseq.