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Updated: Dec 21, 2025

Author Spotlight: Diatom Testing for Forensic Drowning Examination
Published on: November 10, 2023
Evaluation of L/D ratio in a water-related case for the differentiation between drowning and postmortem immersion
Zhigang Li1, Bo Wu1, Xiao Cheng2
1Guangzhou Forensic Science Institute & Key Laboratory of Forensic Pathology, Ministry of Public Security, Baiyun Avenue 1708, Baiyun District, 510000, Guangzhou, China.
Abstract:
It is a difficult task to determine the cause of death for decomposed bodies found in water. Diatom test has been shown to provide supportive evidence for the diagnosis of drowning. According to our previous studies, the ratio of diatom content in lung tissue to diatom content in drowning medium (L/D ratio) is a very useful indicator to distinguish between drowning and postmortem immersion. In this article, we presented a case of a highly decomposed body being recovered from water with no significant findings on its cause of death. We applied the microwave digestion-vacuum filtration-automated scanning electron microscopy method (MD-VF-Auto SEM) to detect diatoms in the organs and suspected drowning medium. In this case, positive results are found in diatom tests of lung tissue, liver tissue and kidney tissue, while the L/D ratio analysis suggested that the victim was more likely to suffer from postmortem immersion. Taking the result of L/R ratio and other findings into consideration, we can eliminate the possibility of drowning. With continuous study, the L/D ratio analysis would be a valuable tool in diagnosis of drowning.
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