Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

7.1K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
7.1K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

933
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
933
SDS-PAGE01:27

SDS-PAGE

32.5K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
32.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Hydrogel dressing integrating FAK inhibition and ROS scavenging for mechano-chemical treatment of atopic dermatitis.

Nature communications·2026
Same author

Tracking Dynamic Variations of Reactive Oxygen Species and Temperature during Ferroptosis-Induced Hepatic Stellate Cell Activation.

ACS nano·2026
Same author

Machine learning-guided design of mechanoadaptive bioglues for multitissue trauma and first-aid applications.

Nature biomedical engineering·2026
Same author

Harnessing thermo-hydrogen coupling with palladium hydride nanoparticles for superior antitumor therapy.

RSC advances·2026
Same author

Blood Mechanical Intelligence: From Force Sensing to Precision Mechanomedicine.

Research (Washington, D.C.)·2026
Same author

AI-enabled wearable microfluidics for next-generation infection monitoring and therapeutics.

Lab on a chip·2026

Related Experiment Video

Updated: Dec 21, 2025

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

7.0K

Liquid Plasticine Integrated with Isoelectric Focusing for Miniaturized Protein Analysis.

Jicheng Niu1,2, Haixiao Shi3, Huigang Wei4

  • 1The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.

Analytical Chemistry
|May 16, 2020
PubMed
Summary

This study introduces liquid plasticine (LP) channels for protein sample pretreatment, enabling rapid, on-site analysis. The developed LP-isoelectric focusing (IEF) platform achieves protein separation and concentration for disease diagnosis.

More Related Videos

Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

12.9K
Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

16.6K

Related Experiment Videos

Last Updated: Dec 21, 2025

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

7.0K
Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

12.9K
Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

16.6K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Miniaturized and rapid protein analytical platforms are crucial for on-site disease diagnosis and monitoring.
  • Liquid marbles (LMs) offer potential as microreactors but face challenges in complex sample pretreatment.
  • Existing LM platforms lack efficient sample preparation capabilities for advanced protein analysis.

Purpose of the Study:

  • To develop a novel liquid plasticine (LP)-isoelectric focusing (IEF) platform for integrated protein separation and concentration.
  • To enable on-site pretreatment of complex protein samples using deformable LMs.
  • To demonstrate the utility of the LP-IEF platform for quantitative protein analysis in clinical samples.

Main Methods:

  • Fabrication of fluid channels using deformable liquid marbles (LMs), termed liquid plasticine (LP).
  • Integration of LP channels with isoelectric focusing (IEF) for simultaneous protein separation and concentration.
  • Application of the LP-IEF platform to standard proteins, human serum, and clinical urine samples.

Main Results:

  • Achieved simultaneous protein separation and 10-fold concentration with a resolution of 0.03 pH.
  • Demonstrated effective sample pretreatment for proteins in physiological samples like human serum.
  • Successfully performed quantitative analysis of microalbuminuria and identified specific proteins in diabetic urine samples.

Conclusions:

  • The developed LP-IEF platform offers a miniaturized and rapid solution for on-site protein analysis.
  • This work establishes LMs/LPs as a versatile strategy for multifunctional integrated analytical platforms.
  • The LP-IEF device shows significant potential for advancing point-of-care diagnostics and protein biomarker discovery.