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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Dec 21, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays.

João L Lagarto^1,2, Federica Villa³, Simone Tisa⁴

¹National Institute of Optics National Research Council (INO-CNR), Largo Enrico Fermi 6, 50125, Florence, Italy. lagarto@lens.unifi.it.

Scientific Reports

|May 17, 2020

Summary

This study introduces a new handheld device using Single Photon Avalanche Diode (SPAD) arrays for real-time autofluorescence lifetime imaging. This technology enables label-free tissue diagnostics by distinguishing between healthy and diseased states.

Failed At:

2026-06-19T13:38:34.831624+00:00

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