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[Technics for B-cell determination in lymphocytes].

H Storch

    Zeitschrift Fur Die Gesamte Innere Medizin Und Ihre Grenzgebiete
    |February 15, 1977
    PubMed
    Summary
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    Determining T- and B-lymphocyte populations in peripheral blood is crucial for diagnosing immunoproliferative disorders, immune defects, and autoimmune diseases. Various methods, including immunofluorescence and rosette tests, aid in this critical diagnostic process.

    Area of Science:

    • Immunology
    • Cell Biology

    Context:

    • The subdivision of lymphocytes into T-cells (thymus-dependent) and B-cells (bone marrow/bursa-dependent) is clinically significant for diagnosing various immune-related conditions.
    • Accurate determination of lymphocyte populations is essential for understanding and managing immunoproliferative disorders, immune defects, and autoimmune diseases.

    Purpose:

    • To highlight the clinical significance of differentiating T- and B-lymphocytes in peripheral blood.
    • To review current methodologies for enumerating T- and B-lymphocytes, including rosette tests and immunofluorescence.
    • To present findings from investigations using immunofluorescence and immune enzyme techniques on patient samples.

    Summary:

    • Established methods for T-lymphocyte determination include E-rosette tests and anti-T-lymphocyte globulin immunofluorescence.

    Related Experiment Videos

  • B-lymphocyte identification utilizes immunofluorescence microscopy, autoradiography, immune enzyme techniques, and rosette tests for Fc and complement receptors.
  • Own investigations confirmed a high proportion of IgM-receptors on normal lymphocytes and observed decreased B-lymphocytes in chronic lymphadenosis patients undergoing cytostatic therapy.

    • Impact:

    • Findings contribute to a better understanding of the pathogenesis, therapeutic interventions, and disease course variations in immunoproliferative and immunodeficient conditions.
    • The study underscores the importance of lymphocyte subset analysis in clinical practice for diagnosis and treatment monitoring.
    • Further research into lymphocyte populations can lead to novel diagnostic and therapeutic strategies for immune-related diseases.