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Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization
Tania Ho-Plágaro1, María Isabel Tamayo-Navarrete1, José Manuel García-Garrido2
1Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (EEZ) CSIC, Granada, Spain.
Abstract:
Histochemical staining and light microscopy-based techniques have been widely used to detect and quantify arbuscular mycorrhizal fungi (AMF) in roots. Here we describe a standardized method for staining of AMF in colonized roots, and we provide possible modifications to adjust the protocol according to particular requirements, such as the type of root material or the reduction of toxic products. In addition, we also summarize some of the most common ways to quantify arbuscular mycorrhizal colonization.
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