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Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
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Single-Spore Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi
1Agroécologie, Université de Bourgogne, Université de Bourgogne Franche-Comté, INRAE, AgroSup Dijon, CNRS, Dijon, France. dirk.redecker@inrae.fr.
Methods in Molecular Biology (Clifton, N.J.)
|May 17, 2020
Abstract:
Biomass of arbuscular mycorrhizal fungi (AMF, Glomeromycota) is often only available in small quantities as these fungi are obligate biotrophs and many species are difficult to cultivate under controlled conditions. Here, I describe a simple, efficient approach to produce crude extracts from single or a small number of spores that can be used for genotyping AMF.

