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Updated: Dec 21, 2025

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DrawAlignR: An Interactive Tool for Across Run Chromatogram Alignment Visualization
Shubham Gupta1,2, Justin Sing1,2, Arshia Mahmoodi2,3
1Department of Molecular Genetics, University of Toronto, Toronto, ON, M5G 1A8, Canada.
Abstract:
Multi-run alignment is widely used in proteomics to establish analyte correspondence across runs. Generally alignment algorithms return a cumulative score, which may not be easily interpretable for each peptide. Here a novel and interactive tool for cross-run chromatogram alignment visualization (DrawAlignR) of data-independent acquisition (DIA) data is presented. Furthermore, a novel C++ based implementation of raw chromatogram alignment which is 35 times faster than the previously published algorithm is developed. This not only enables users to plot alignment interactively by DrawAlignR, but also allows other software platforms to use the algorithm. DrawAlignR is an open-source web application using R Shiny that can be hosted using the source-code available at https://github.com/Roestlab/DrawAlignR.
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