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Updated: Dec 21, 2025

Mouse Adipose Tissue Collection and Processing for RNA Analysis
Published on: January 31, 2018
The use of an inexpensive processing aid device (the Mouse PAD) to facilitate rodent tissue banking
Zachary D Brown1, Muath Bishawi2,3, Jun-Neng Roan2,4
1Division of Surgical Sciences, Department of Surgery, Duke University, Durham, NC 27710, USA.
Abstract:
The generation of an experimental animal model often requires considerable investment of both time and money. Typically, investigators are interested in specific organs and when experimental animals are euthanized, tissues that are not the focus of the research project are discarded. However, the remaining tissues from these animals could provide valuable scientific information if efficient, error-proof and economical approaches to collect and biobank them were available. We have developed a device that, when incorporated into our tissue processing workflow, allows for high-throughput collection and processing of multiple rodent organ systems. This device, the mouse Processing Aid Device, or mouse PAD, helps to standardize organ collection and increase its efficiency.

