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Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

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Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform.

Jacob Berger^1,2,3, Enrique Valera^1,2,3, Aaron Jankelow^1,2,3

¹Department of Bioengineering, University of Illinois at Urbana-Champaign, 1102 Everitt Lab, MC 278, 1406 W. Green St, Urbana, IL, 61801, USA.

Biomedical Microdevices

|May 19, 2020

Summary

This study introduces a novel point-of-care system for rapid sepsis biomarker detection. The microfluidic device accurately quantifies Interleukin-6 (IL-6) and Procalcitonin (PCT) in patient plasma, aiding sepsis diagnosis.

Keywords:

IL-6 and PCT Micro-bead assay Microfluidic electrical impedance Point-of-care diagnostics Protein detection Sepsis

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Area of Science:

Biomedical Engineering
Clinical Diagnostics
Biomarker Analysis

Background:

Sepsis is a leading cause of mortality and healthcare costs in the U.S.
Current sepsis diagnosis relies on non-specific clinical parameters.
Biomarkers like Interleukin-6 (IL-6) and Procalcitonin (PCT) show promise for sepsis management.

Purpose of the Study:

To develop and validate a microfluidic point-of-care (POC) platform for simultaneous detection of IL-6 and PCT.
To correlate biomarker concentrations with sepsis severity using advanced analytics.
To improve sepsis diagnosis and prognosis through rapid, multi-parameter testing.

Main Methods:

Utilized impedance cytometry, microbead immunoassays, and biotin-streptavidin binding.
Developed a multiplexed immunoassay for simultaneous IL-6 and PCT detection in human plasma.
Validated the POC system by quantifying biomarker concentrations in samples with varying levels (healthy, medium, high).

Main Results:

The microfluidic POC system successfully correlated microbead capture to IL-6 and PCT concentrations.
Demonstrated statistically significant (P < 0.05 to P < 0.00001) detection of IL-6 and PCT across different concentration levels.
Validated the device's capability for simultaneous, quantitative measurement of key sepsis biomarkers.

Conclusions:

The developed microfluidic POC platform enables rapid, simultaneous detection of sepsis biomarkers IL-6 and PCT.
This technology has the potential to significantly aid in the early diagnosis and prognosis of sepsis patients.
The system's validation supports its application as a POC tool for improving sepsis management strategies.