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Updated: Dec 21, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Conjugating pyrene onto PNA-based fluorescent probes for improved detection selectivity toward double-stranded siRNA
Yusuke Sato1, Yuki Takahashi1, Takaaki Tanabe1
1Department of Chemistry, Graduate School of Science, Tohoku University, Japan, Sendai 980-8578, Japan. yusuke.sato.a7@tohoku.ac.jp seiichi.nishizawa.c8@tohoku.ac.jp.
Abstract:
We report on the design of new siRNA-binding fluorescent probes with the improved detection selectivity toward intact double-stranded siRNAs over single-stranded forms by the conjugation of pyrene unit into thiazole orange base surrogate-carrying peptide nucleic acid (PNA) that can simultaneously recognize the 3'-overhang and double-stranded sequences of target siRNAs.

