Hymenobacter setariae sp. nov., isolated from the ubiquitous weedy grass Setaria viridis

A Gram-stain-negative, short-rod, aerobic, non-motile, red to pink-pigmented bacterium, designated Fur1^T, was isolated from the dry spikelet clusters of a plant called Setaria viridis near Dongguk University. Phylogenetic analysis conducted based on 16S rRNA gene sequences indicated that strain Fur1^T belonged to the genus Hymenobacter of the family Hymenobacteraceae. The 16S rRNA gene of Fur1^T showed highest sequence similarity to those of Hymenobacter metalli KACC 17381^T (97.5 %) and Hymenobacter marinus KACC 19042^T (97.1 %). Growth occurred at 4-37 °C (optimum, 25-28 °C), up to 1.0 % NaCl (optimum, 0 %) and pH 5.5-9.0 (optimum, pH 6.0-7.5). The major fatty acids of strain Fur1^T were identified as iso-C_15 : 0, C_16 : 1  ω5c, anteiso-C_15 : 0, summed feature 3 (comprising C_16 : 1  ω7c and/or C_16 : 1  ω6c) and summed feature 4 (comprising anteiso-C_17 : 1B and/or iso-C_17 : 1I) as the major cellular fatty acids. The predominant respiratory quinone was identified as MK-7. The polar lipids were phosphatidylethanolamine, five unidentified aminophospholipids, two unidentified phospholipids, one unidentified glycolipid and one unidentified polar lipid. The genomic DNA G+C content based on the draft genome sequence was 58.7 mol%. DNA-DNA relatedness between strain Fur1^T and its closest relative was below 70 %. Characterization based on phylogenetic, chemotaxonomic and phenotypic analyses clearly indicated that strain Fur1^T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter setariae sp. nov. is proposed. The type strain is Fur1^T (=KACC 19903^T=NBRC=113691^T).