Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.6K
Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K
Subcellular Fractionation01:32

Subcellular Fractionation

8.5K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
8.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tissue-specific CTG•CAG expansion rate and disease severity are modified by DNA repair genes expression levels in myotonic dystrophy type 1 patients.

DNA repair·2026
Same author

A new era for myotonic dystrophy: Improved delivery of a DMPK-targeted oligonucleotide modulates muscle transcriptomes and function.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Muscleblind-like proteins dimerize by forming disulfide bonds to regulate alternative splicing and pathogenic RNA foci formation.

Nucleic acids research·2026
Same author

Enhanced antisense oligonucleotide delivery reveals that transcript turnover impacts apparent splicing rescue in myotonic dystrophy.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Myonuclear Domain-Associated and Central Nucleation-Dependent Spatial Restriction of Dystrophin Protein Expression.

Journal of cachexia, sarcopenia and muscle·2026
Same author

Muscleblind-like proteins dimerize by forming disulfide bonds to regulate alternative splicing and pathogenic RNA foci formation.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Dec 21, 2025

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

544

Transcriptome-wide organization of subcellular microenvironments revealed by ATLAS-Seq.

Danielle A Adekunle1,2, Eric T Wang1

  • 1Department of Molecular Genetics & Microbiology, UF Genetics Institute, Center for NeuroGenetics, University of Florida, USA.

Nucleic Acids Research
|May 19, 2020
PubMed
Summary

Researchers developed ATLAS-Seq to map RNA and protein locations within cells. This technique reveals that RNAs often cluster by function and protein complexes, not just by their encoded proteins, offering new insights into cellular organization.

More Related Videos

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

3.2K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

19.0K

Related Experiment Videos

Last Updated: Dec 21, 2025

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

544
Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

3.2K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

19.0K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genomics

Background:

  • Subcellular organization of RNAs and proteins is crucial for cellular function.
  • Current understanding of molecular localization within cells and tissues remains incomplete.
  • Global maps and conceptual frameworks for RNA and protein localization are needed.

Purpose of the Study:

  • To introduce ATLAS-Seq, a novel method for mapping RNA and protein localization.
  • To generate transcriptomes and proteomes from fractionated tissue lysates.
  • To investigate the principles governing subcellular organization of RNA and protein molecules.

Main Methods:

  • Utilized detergent-free tissue lysates fractionated via sucrose gradient centrifugation.
  • Performed proteomic analysis to confirm separation of subcellular compartments.
  • Generated transcriptomic and proteomic data from fractionated samples.

Main Results:

  • Proteomic analysis validated the separation of subcellular compartments.
  • RNAs unexpectedly co-sedimented with other RNAs, protein complexes, or functionally related molecules.
  • Most RNAs showed different sedimentation profiles compared to their encoded proteins, except for those of secreted proteins.
  • Hundreds of alternative RNA isoforms displayed distinct sedimentation patterns.
  • Identified potential RNA binding proteins driving observed RNA localization patterns.
  • Confirmed known RNA-protein interactions and predicted novel associations.

Conclusions:

  • RNAs are organized into networks of co-segregating mRNAs encoding functionally related proteins.
  • These findings provide new insights into the establishment and maintenance of subcellular organization.
  • ATLAS-Seq offers a powerful tool for dissecting molecular organization within cells.