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Updated: Dec 21, 2025

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
Published on: December 12, 2017
CRISPR/CAS9-Mediated Antiviral Activity: A Tool to Combat Viral Infection
Usman Ali Ashfaq1, Hina Khalid2
1Department of Bioinformatics & Biotechnology, Government College University, 38000 Faisalabad, Pakistan.
Abstract:
Viruses hijack host cellular receptors and functions for replication, thereby posing a complication in identifying therapeutic targets. The CRISPR/Cas 9 platform has revolutionized gene-editing modalities in a wide range of cells and organisms with high potential in therapeutics. Recently, it has been put to work targeting human pathogenic viruses that interrupt receptors and functions with viral replication. This review encompasses major discoveries in CRISPR/Cas as an antiviral strategy. Additionally, challenges that demand consideration prior to its use in the clinic as part of the antiviral armamentarium are briefly addressed.
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