Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.0K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Transcription01:17

Transcription

32.0K
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
32.0K
Transcription01:10

Transcription

154.6K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
154.6K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

25.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.5K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.7K
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation, Characterization, and Self-Assembly of P3HT-Based Janus Fibers via a Crystallization-Driven Self-Assembly Process.

ACS macro letters·2025
Same author

Dynamic landscape and regulation of m<sup>5</sup>C methylation in human tissues.

Science China. Life sciences·2025
Same author

Underlying Spatial Diversity Patterns of Freshwater Crabs in Southern China, With Recommendations for Conservation of Freshwater Biodiversity.

Ecology and evolution·2025
Same author

Climate-Induced Range Shift and Risk Assessment of Emerging Weeds in Queensland, Australia.

Ecology and evolution·2025
Same author

Advancing digital health in China: Aligning challenges, opportunities, and solutions with the Global Initiative on Digital Health (GIDH).

Health care science·2024
Same author

Development of a carboxymethyl chitosan functionalized slide for small molecule detection using oblique-incidence reflectivity difference technology.

Biomedical optics express·2024

Related Experiment Video

Updated: Dec 21, 2025

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

9.2K

RNA structural dynamics regulate early embryogenesis through controlling transcriptome fate and function.

Boyang Shi1,2, Jinsong Zhang3, Jian Heng2,4

  • 1CAS Key Laboratory of Genomic and Precision Medicine, Collaborative Innovation Center of Genetics and Development, College of Future Technology, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China.

Genome Biology
|May 20, 2020
PubMed
Summary
This summary is machine-generated.

RNA structure in zebrafish embryogenesis impacts maternal mRNA fate. Elavl1a protein stabilizes maternal RNAs, but RNA structure formation triggers their decay, revealing a key regulatory role.

Keywords:
Elavl1aRNA structureStructure-based regulomeZebrafish early embryogenesis

More Related Videos

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.5K
Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
06:49

Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

Published on: January 12, 2022

3.6K

Related Experiment Videos

Last Updated: Dec 21, 2025

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

9.2K
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.5K
Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
06:49

Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

Published on: January 12, 2022

3.6K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Vertebrate early embryogenesis relies on maternal RNAs and proteins, but regulatory mechanisms are unclear.
  • The role of RNA structure in maternal transcriptome regulation remains largely undetermined.
  • Recent studies highlight RNA structure's importance in RNA processing and function.

Purpose of the Study:

  • To map mRNA structures during zebrafish early embryogenesis.
  • To investigate how RNA structure influences maternal mRNA fate and the maternal-to-zygotic transition (MZT).
  • To elucidate the role of RNA-binding proteins in structure-dependent RNA regulation.

Main Methods:

  • Global mapping of mRNA structures using icSHAPE in zebrafish embryos.
  • Analysis of RNA structurally variable regions and their enrichment in 3' UTRs.
  • Investigating the interaction between RNA-binding protein Elavl1a and maternal mRNAs.

Main Results:

  • RNA structurally variable regions are enriched in 3' UTRs and contain cis-regulatory elements crucial for MZT.
  • The RNA-binding protein Elavl1a stabilizes maternal mRNAs by binding to cis-elements.
  • RNA structure formation inhibits Elavl1a binding, leading to maternal target mRNA decay.

Conclusions:

  • RNA structural variability is prevalent in 3' UTRs and plays a role in zebrafish early development.
  • Elavl1a regulates maternal RNA stability in an RNA structure-dependent manner.
  • RNA structure-based regulation is fundamental to vertebrate early embryogenesis.