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Related Experiment Videos

Immunoelectron microscopic study of thyroid microsomal antigen.

S Yokoyama1, I Nakayama, S Noguchi

  • 1Department of Pathology, Medical College of Oita, Japan.

Acta Pathologica Japonica
|September 1, 1988
PubMed
Summary
This summary is machine-generated.

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Thyroid microsomal antigen (TMAg) is exclusively located on the apical plasma membrane of thyroid follicular cells. This finding was determined using electron microscopy in patients with Basedow's disease.

Area of Science:

  • Immunology
  • Cell Biology
  • Endocrinology

Background:

  • Thyroid microsomal antigen (TMAg) is a key autoantigen in autoimmune thyroid diseases like Graves' disease (Basedow's disease).
  • The precise cellular localization of TMAg is crucial for understanding autoimmune responses in the thyroid.

Purpose of the Study:

  • To determine the specific localization of thyroid microsomal antigen (TMAg) on thyroid follicular cells using immunoelectron microscopy.
  • To investigate the role of spontaneous membrane-bound IgG in the observed antigen localization.

Main Methods:

  • Immunoelectron microscopy was employed on dissociated follicular cells from patients with Basedow's disease.
  • Human IgG F(ab')2 fragments from pooled sera with high thyroid microsomal antibody (TMAb) titers were used.

Related Experiment Videos

  • Spontaneous membrane-bound IgG was masked using anti-human IgG-goat IgG Fab to differentiate antigen localization.

    • Main Results:

    • Spontaneous membrane-bound IgG was detected on the apical plasma membrane in most TMAb-positive cases.
    • After masking, intense TMAg reaction product was observed linearly on the apical plasma membrane of numerous follicular cells.
    • No TMAg signal was detected on lateral/basal membranes or within the cytoplasm.

    Conclusions:

    • Thyroid microsomal antigen (TMAg) is definitively localized to the apical plasma membrane of thyroid follicular cells.
    • This apical localization provides insights into the target of autoimmune attacks in conditions like Basedow's disease.