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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
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Published on: March 14, 2019

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Circular RNA hsa_circ_0008285 inhibits colorectal cancer cell proliferation and migration via the miR-382-5p/PTEN

Jianjun Wang1, Juanjuan Luo2, Gan Liu3

  • 1Department of Histology and Embryology, Wannan Medical College, Wuhu, 241002, China.

Biochemical and Biophysical Research Communications
|May 20, 2020
PubMed

Insights

Circular RNA hsa_circ_0008285 is downregulated in colorectal cancer (CRC), promoting tumor growth. It suppresses the PI3K/AKT pathway via miR-382-5p/PTEN, indicating its potential as a CRC therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in various cancers, but their specific roles and mechanisms in colorectal cancer (CRC) remain underexplored.
  • Hsa_circ_0008285 (circ_0008285), derived from the CDYL gene, shows differential expression in cancers, being upregulated in some but reduced in colon cancer.

Purpose of the Study:

  • To investigate the function and molecular mechanism of circ_0008285 in the progression of colorectal cancer (CRC).
  • To evaluate circ_0008285 as a potential therapeutic target for CRC.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to confirm circ_0008285 expression in CRC tissues and cell lines.
  • Clinicopathological analysis to correlate circ_0008285 levels with tumor progression.
  • In vitro functional assays (e.g., knockdown) to assess effects on CRC cell proliferation and migration.
  • RNA-sequencing, bioinformatics, dual-luciferase reporter assays, and western blotting to elucidate the underlying molecular mechanism.

Main Results:

  • Circ_0008285 expression was significantly downregulated in CRC tissues and cell lines compared to normal controls.
  • Lower circ_0008285 levels correlated inversely with tumor size, lymphatic metastasis, and TNM stage.
  • Knockdown of circ_0008285 enhanced CRC cell proliferation and migration in vitro.
  • Mechanistically, circ_0008285 was found to suppress the PI3K/AKT signaling pathway through the miR-382-5p/PTEN axis.

Conclusions:

  • Circ_0008285 acts as a tumor suppressor in colorectal cancer.
  • The findings suggest that circ_0008285 could be a potential diagnostic biomarker and therapeutic target for CRC treatment.
Keywords:
Circular RNAColorectal cancerHsa_circ_0008285PTENmiR-382-5p

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