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Related Experiment Video

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In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
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In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster

Published on: June 13, 2025

1000

Split-TurboID enables contact-dependent proximity labeling in cells.

Kelvin F Cho1, Tess C Branon2,3,4,5, Sanjana Rajeev2

  • 1Cancer Biology Program, Stanford University, Stanford, CA 94305.

Proceedings of the National Academy of Sciences of the United States of America
|May 20, 2020
PubMed
Summary

Researchers developed split-TurboID, a novel proximity labeling tool. This method enables the study of challenging cellular regions like organelle contact sites, identifying new proteins involved in these interactions.

Keywords:
ER–mitochondria contactsproximity labelingsplit-TurboID

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Area of Science:

  • Cell Biology
  • Proteomics
  • Biochemistry

Background:

  • Proximity labeling (PL) using enzymes like TurboID aids proteomic analysis of difficult-to-access subcellular regions.
  • Conventional methods struggle to analyze specific cellular compartments, such as organelle contact sites.

Purpose of the Study:

  • To develop a proximity labeling method capable of analyzing organelle contact sites.
  • To overcome limitations of current PL techniques for studying specific subcellular interactions.

Main Methods:

  • Engineered a split-TurboID enzyme, comprising two inactive fragments that reconstitute upon protein-protein interaction or membrane apposition.
  • Applied split-TurboID to endoplasmic reticulum-mitochondria contact sites for spatially restricted biotinylation.
  • Utilized mass spectrometry for enrichment and identification of biotinylated proteins.

Main Results:

  • Successfully identified over 100 endogenous proteins at endoplasmic reticulum-mitochondria contact sites.
  • Discovered numerous proteins not previously associated with these organelle contacts.
  • Validated eight candidate proteins using biochemical fractionation and overexpression imaging.

Conclusions:

  • Split-TurboID is a versatile tool for conditional and spatially specific proximity labeling.
  • Enables the proteomic investigation of previously inaccessible cellular regions.
  • Expands the toolkit for studying organelle interactions and cellular organization.