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Related Concept Videos

Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding.

Yujie Xie1,2, Maria C Arno1, Jonathan T Husband1

  • 1School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.

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|May 20, 2020
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Summary

We developed a photo-switchable nanogel for microscale information storage. This system uses light to control fluorescence lifetime, enabling reversible data encoding and readout with fluorescence lifetime imaging microscopy (FLIM).

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Area of Science:

  • Biotechnology
  • Materials Science
  • Microscopy

Background:

  • Fluorescent barcoding is crucial for microscale research, but fluorescence lifetime offers superior quantitative and reproducible data.
  • Current fluorescence lifetime applications are limited by the lack of spatiotemporal control over the coding process.
  • Developing methods for precise manipulation of fluorescence lifetime is essential for advancing microscale investigations.

Purpose of the Study:

  • To design a novel two-component photo-switchable nanogel system.
  • To enable spatiotemporal manipulation of fluorescence lifetime for microscale applications.
  • To demonstrate the utility of this system for information storage and background reduction in live-cell imaging.

Main Methods:

  • Fabrication of a two-component photo-switchable nanogel.
  • Induction of variable fluorescence lifetime via photoisomerization-induced energy transfer using light irradiation.
  • Visualization and mapping of fluorescence lifetime using fluorescence lifetime imaging microscopy (FLIM).

Main Results:

  • The nanogel demonstrated tunable fluorescence lifetime properties upon light-induced photoisomerization.
  • Remote manipulation and visual mapping of fluorescence lifetime were achieved using FLIM.
  • The system exhibited reversibility, allowing for dynamic information storage and display at the microscale.

Conclusions:

  • The photo-switchable nanogel offers a novel platform for spatiotemporal control of fluorescence lifetime.
  • This technology enables advanced microscale information storage and dynamic process monitoring.
  • The system's reversibility is valuable for reducing background noise in fluorescence lifetime imaging of biological samples.