Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Discovery: Overview01:26

Drug Discovery: Overview

10.7K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
10.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantification of a serum titin fragment reflects dystrophin restoration in mdx mice and disease severity in patients with dystrophinopathies.

Journal of neuromuscular diseases·2026
Same author

The rise and fall of SARM1 base-exchange inhibitors.

Communications chemistry·2026
Same author

A phase 1 clinical trial of a multi-antigen SARS-CoV DNA vaccine as a booster after dose three spike-based mRNA vaccinations.

Molecular therapy. Advances·2026
Same author

Molecular Basis of c‑MET Inhibition by Approved Small Molecule Drugs: A Structural Perspective.

ACS medicinal chemistry letters·2026
Same author

RP3Net: a deep learning model for predicting recombinant protein production in Escherichia coli.

Bioinformatics (Oxford, England)·2026
Same author

Engineered calcium-regulated affinity protein for efficient internalization and lysosomal toxin delivery.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Dec 21, 2025

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.9K

Secretome-Based Screening in Target Discovery.

Mei Ding1, Hanna Tegel2, Åsa Sivertsson2

  • 1Discovery Biology, Discovery Sciences, R&D, AstraZeneca, Gothenburg, Sweden.

SLAS Discovery : Advancing Life Sciences R & D
|May 20, 2020
PubMed
Summary

Secretome libraries enable rapid discovery of novel drug targets by identifying active proteins and their receptors. This approach accelerates understanding of disease pathways for drug discovery.

Keywords:
cell-based screeningphenotypic drug discoverysecretome librarytarget discovery

More Related Videos

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
10:43

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Published on: May 24, 2014

11.7K
Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.7K

Related Experiment Videos

Last Updated: Dec 21, 2025

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.9K
siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
10:43

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Published on: May 24, 2014

11.7K
Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.7K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Secreted proteins and plasma membrane receptors mediate intercellular communication, regulating human physiology.
  • Secretome-based screening platforms facilitate the discovery of novel biological insights and signaling pathways.
  • These platforms are valuable for target identification in drug discovery.

Purpose of the Study:

  • To review the current status and applications of secretome-based screening.
  • To present a workflow for secretome-based screening based on industry experience.
  • To highlight the advantages of secretome libraries for phenotypic target discovery.

Main Methods:

  • Summarizing the annotation, production, quality control, and sample management of secretome libraries.
  • Reviewing the application of secretome libraries in disease-relevant cell-based assays.
  • Sharing a practical workflow for secretome-based screening.

Main Results:

  • Secretome libraries enable direct identification of active proteins and their cognate receptors.
  • This approach facilitates rapid understanding of disease pathways and target hypothesis generation.
  • Secretome libraries cover significant biological signaling space and are suitable for physiologically relevant cell types.

Conclusions:

  • Secretome-based screening is an attractive approach for phenotypic target discovery.
  • The direct identification of targets and receptors accelerates drug discovery pipelines.
  • Secretome libraries offer a powerful tool for exploring novel biology and therapeutic targets.