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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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Circular RNA Expression Profiles in Nasopharyngeal Carcinoma by Sequence Analysis
Jing Yang1, Yongqian Gong2, Qingshan Jiang2
1Department of Gastroenterology, The First Affiliated Hospital of University of South China, Hengyang, China.
Frontiers in Oncology
|May 20, 2020
Summary
This study identifies novel circular RNA (circRNA) expression profiles in nasopharyngeal carcinoma (NPC). Key circRNAs, like circKITLG, were found to influence NPC progression by interacting with microRNAs, offering potential therapeutic targets.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Circular RNAs (circRNAs) are emerging non-coding RNAs regulating gene expression.
- Nasopharyngeal carcinoma (NPC) is a significant head and neck cancer with complex molecular underpinnings.
Purpose of the Study:
- To investigate the circRNA expression profile in nasopharyngeal carcinoma (NPC).
- To identify specific circRNAs involved in NPC pathogenesis and their potential mechanisms.
Main Methods:
- High-throughput sequencing to compare circRNA expression between NPC patients and healthy controls.
- Quantitative real-time PCR (qRT-PCR) for validation of differentially expressed circRNAs.
- Gene Ontology (GO), KEGG pathway, and circRNA-miRNA-target gene network analyses.
Main Results:
- Identified 93 upregulated and 77 downregulated circRNAs in NPC.
- Validated key circRNAs, including circKITLG, showing significant differential expression.
- Discovered that circKITLG may sponge miR-3198, impacting NPC cell proliferation, migration, and invasion.
Conclusions:
- This study establishes a foundational circRNA expression profile for NPC.
- circKITLG emerges as a potential oncogenic circRNA in NPC, targeting the miR-3198 pathway.
- These findings offer novel insights into NPC molecular mechanisms and potential therapeutic strategies.
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