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Updated: Dec 21, 2025

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Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
Published on: March 23, 2020
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Monitoring protein ubiquitination and SUMOylation in real-time by NMR
Batul Ismail Habibullah1, Vasvi Tripathi1, Parag Surana1
1National Centre for Biological Sciences, Bengaluru-560065, India. rana@ncbs.res.in.
Abstract:
The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers.

