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Updated: Dec 21, 2025

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
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Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Published on: July 6, 2012

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mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding

Chen Bao1, Sarah Loerch2, Clarence Ling1

  • 1Department of Biochemistry and Biophysics at School of Medicine and Dentistry and Center for RNA Biology, University of Rochester, Rochester, United States.

Elife
|May 20, 2020
PubMed

Keywords:
E. colichromosomescryo-EMgene expressionmRNAmolecular biophysicsribosomesmFRETstructural biologytranslation

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