Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-omics analysis of type II diabetic wound healing reveals CD44-mediated immune cell crosstalk dysfunction in mice and humans.

bioRxiv : the preprint server for biology·2026
Same author

Examining neuroimaging biomarkers, plasma biomarkers and cognitive functions in patients with recovered COVID-19 infection: a multicentre study using 7T MRI.

Brain communications·2026
Same author

<i>Ixodes scapularis</i> STING promotes Powassan virus infection in ticks.

Research square·2026
Same author

Biomarkers.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Association between Vitamin B12 Levels and Colon Cancer Survival: A Global Network Study.

Cancer research communications·2026
Same author

Biomarkers.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Related Experiment Video

Updated: Dec 21, 2025

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

10.0K

Measuring Alphavirus Fidelity Using Non-Infectious Virus Particles.

Edward I Patterson1,2, Kamil Khanipov3, Daniele M Swetnam4

  • 1Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.

Viruses
|May 21, 2020
PubMed
Summary

Altering viral mutation rates can create live-attenuated vaccines. Researchers deleted a VEEV cleavage site to study mutations without selection, revealing lower mutation frequencies in engineered viruses.

Keywords:
Alphavirusarbovirusfidelity mutantsmutation frequency

More Related Videos

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

13.3K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.6K

Related Experiment Videos

Last Updated: Dec 21, 2025

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

10.0K
Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

13.3K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.6K

Area of Science:

  • Virology
  • Vaccine Development
  • Molecular Biology

Background:

  • RNA viruses incorporate mutations at an optimal frequency, crucial for live-attenuated vaccine strategies.
  • Assessing the impact of specific mutations on viral fidelity has been challenging due to rapid selection during replication.

Purpose of the Study:

  • To develop a method for evaluating the effect of mutations on viral mutation frequency without the confounding factor of selection.
  • To investigate the role of the PE2 cleavage site in Venezuelan equine encephalitis virus (VEEV) fidelity.

Main Methods:

  • Deletion of structural polyprotein PE2 cleavage site residues (E3D56-59) in VEEV TC-83 to create non-infectious particles.
  • Utilizing next-generation sequencing to analyze mutation frequencies in engineered VEEV particles.

Main Results:

  • Deletion of the PE2 cleavage site rendered VEEV non-infectious, enabling mutation analysis without selection.
  • Fidelity mutants exhibited significantly lower transversion and overall mutation frequencies compared to the control VEEV TC-83 E3D56-59.

Conclusions:

  • The PE2 cleavage site deletion strategy effectively isolates the effect of mutations on viral fidelity.
  • This method provides a platform for evaluating fidelity mutants across medically important alphaviruses.