Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immune-driven stromal inflammation in pancreatic cancer within a microfluidic platform.

Biofabrication·2026
Same author

Multipopulation Genome-Wide Association Study Identifies Novel Loci for Bicuspid Aortic Valve and Reveals Shared Genetic Architecture With Aortopathies.

Circulation. Genomic and precision medicine·2026
Same author

A Novel SEM Image Processing Approach for Evaluating Sterilization Effects on Polymeric Medical Devices: Validation Against Traditional EDX Analysis.

Polymers·2025
Same author

INSM1 governs a neuronal progenitor state that drives glioblastoma in a human stem cell model.

Nature communications·2025
Same author

Microfluidic device for islet conformal coating with a polyethylene glycol-based hydrogel: innovating cell immunoprotection strategies in type 1 diabetes.

Lab on a chip·2025
Same author

A Metrics-Driven Approach to Develop a Hybrid Model of Staffing and Workload Balance in the NGHA Hospitals.

Journal of healthcare leadership·2025

Related Experiment Video

Updated: Dec 21, 2025

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
12:33

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Published on: June 25, 2014

65.9K

Microelectrode Array based Functional Testing of Pancreatic Islet Cells.

Ahmad Alassaf1,2,3, Matthew Ishahak1,2, Annie Bowles1,2

  • 1Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, USA.

Micromachines
|May 21, 2020
PubMed
Summary
This summary is machine-generated.

Microelectrode arrays (MEAs) enable real-time, non-invasive electrophysiology of islet cells. This method effectively assesses islet functionality and glucose response over extended culture periods.

Keywords:
electrochemical transductioninsulin secretionislets of Langerhansmicroelectrode array (MEA), glucose stimulated insulin response

More Related Videos

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

2.4K
Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis
07:58

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis

Published on: March 9, 2022

1.8K

Related Experiment Videos

Last Updated: Dec 21, 2025

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
12:33

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Published on: June 25, 2014

65.9K
Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

2.4K
Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis
07:58

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis

Published on: March 9, 2022

1.8K

Area of Science:

  • Endocrinology
  • Neuroscience
  • Biomedical Engineering

Background:

  • Islet of Langerhans functionality assessment is crucial for diabetes research.
  • Current electrophysiological methods like patch clamp are limited to short-term, one-time recordings.
  • A need exists for prolonged, real-time, non-invasive techniques to study islet cell electrophysiology.

Purpose of the Study:

  • To describe and validate the use of microelectrode arrays (MEAs) for characterizing the electrophysiology of dissociated human islet cells.
  • To assess islet cell electrical activity in response to glucose over prolonged culture periods (up to 7 days).
  • To compare MEA recordings with glucose-stimulated insulin secretion (GSIS) assays.

Main Methods:

  • Human islets were dissociated into single cells and cultured on MEAs for up to 7 days.
  • Immunofluorescent imaging confirmed the presence of key islet cell subtypes (β, δ, γ).
  • MEA recordings captured electrical activity under low (1.1 mM) and high (16.7 mM) glucose conditions; Fraction of Plateau Phase (FOPP) was analyzed.

Main Results:

  • MEA recordings showed significantly higher electrical activity in high glucose compared to low glucose across all culture days.
  • The FOPP consistently demonstrated greater spiking activity under high glucose conditions.
  • Insulin secretion diminished after day 3 of culture, correlating with MEA spiking profiles.

Conclusions:

  • Extracellular recordings using MEAs provide an effective, real-time method for assessing dissociated islet cell functionality.
  • MEA analysis of islet cell electrophysiology correlates with glucose-stimulated insulin secretion.
  • MEA technology can supplement traditional assays for evaluating islet function.