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Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
Published on: November 9, 2017
Inactivation of the alpha-actinin gene in Dictyostelium
1Max-Planck-Institut fuer Biochemie, Martinsried, Federal Republic of Germany.
Abstract:
alpha-Actinin-negative transformants of Dictyostelium have been obtained by transforming cells with a transformation vector carrying part of the alpha-actinin gene in either sense or antisense orientation. The transformants did not produce detectable alpha-actinin anymore and contained an altered RNA lacking the 3' part of the coding sequences. The deficiency in alpha-actinin was due to an integration of the transformation vector into the gene, since it could be detected by Southern blot analysis in the endogenous gene.
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