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Updated: Dec 21, 2025

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens
Nour Zahi Gammoh1, Lothar Rink2
1Institute of Immunology, Faculty of Medicine, RWTH Aachen University, University Hospital, Aachen, Germany.
Abstract:
Conventional PCR techniques are laborious and usually not suited for fast screening of large sample numbers in a clinical or research setting. Using this closed-tube multiplex real-time PCR, the presence of all 11 Streptococcus pyogenes superantigen (SAg) genes can be rapidly and accurately characterized. Identifying whether a strain contains a SAg can be done within 4 h compared to conventional methods which would take 11 times as long. This method provides an excellent diagnostic tool as well as a screening tool to help researchers clarify the role of SAgs in S. pyogenes infections.

