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Updated: Dec 21, 2025
Bioluminescent Bacterial Imaging In Vivo
Published on: November 4, 2012
Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging
Jacelyn M S Loh1, Kar Yan Soh2, Thomas Proft3
1Department of Molecular Medicine and Pathology, School of Medical Sciences and Maurice Wilkins Centre for Biomolecular Discovery, The University of Auckland, Auckland, New Zealand. mj.loh@auckland.ac.nz.
Abstract:
Bioluminescence is a rapid and cost-saving technology that enables monitoring of bacteria in real time in animal infection models. Here, we report a method for labeling GAS with a set of plasmids we have developed and deposited with Addgene. These plasmids can be used to either integrate firefly luciferase (Ffluc) or red-shifted firefly luciferase (FflucRT) into the GAS genome or to introduce the reporters on plasmids which have been stabilized with a toxin-antitoxin system to prevent loss of plasmid in the absence of antibiotic selection.
