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A large travel-associated outbreak of iatrogenic botulism in four European countries following intragastric botulinum neurotoxin injections for weight reduction, Türkiye, February to March 2023.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2023

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Comprehensive Mass Spectrometric Survey of Streptococcus pyogenes Subcellular Proteomes.

Journal of proteome research·2017

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LL-37-induced host cell cytotoxicity depends on cellular expression of the globular C1q receptor (p33).

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Vigilant keratinocytes trigger pathogen-associated molecular pattern signaling in response to streptococcal M1 protein.

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Protein dynamics tunes excited state positions in light-harvesting complex II.

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Direct interaction of the major light-harvesting complex II and PsbS in nonphotochemical quenching.

Proceedings of the National Academy of Sciences of the United States of America·2013

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Updated: Dec 21, 2025

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

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Protocol for Proteome Analysis of Group A Streptococcus.

¹Division of Infection Medicine, Lund University, Lund, Sweden. wilkl@rki.de.

Methods in Molecular Biology (Clifton, N.J.)

|May 21, 2020

Summary

This study presents a method to separate Streptococcus pyogenes cell fractions for mass spectrometry analysis. This technique aids in identifying potential drug and vaccine targets, especially membrane proteins, for new antimicrobial therapies.

Keywords:

Bacterial proteome Cell wall Cellular fractionation Gram-positive bacteria Mass spectrometry Membrane Streptococcus pyogenes secretome

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Area of Science:

Microbiology
Proteomics
Biochemistry

Background:

Streptococcus pyogenes is a significant human pathogen.
Understanding its subcellular proteomes is crucial for developing novel therapeutics.
Targeting specific cell fractions, like the plasma membrane, offers promising avenues for antimicrobial drug and vaccine development.

Purpose of the Study:

To describe a protocol for separating cell fractions from Streptococcus pyogenes.
To prepare these fractions for mass spectrometry-based proteomic analysis.
To facilitate the identification of potential drug and vaccine targets within S. pyogenes.

Main Methods:

Development of a cell fractionation protocol for Streptococcus pyogenes.
Sample preparation techniques for mass spectrometry.
Analysis of subcellular proteomes using mass spectrometry.

Main Results:

A reproducible protocol for separating Streptococcus pyogenes cell fractions was established.
The method allows for comprehensive analysis of subcellular proteomes.
Identification of proteins, particularly those integral to the plasma membrane, is enabled.

Conclusions:

The described protocol is effective for analyzing Streptococcus pyogenes subcellular proteomes.
This approach can identify novel drug and vaccine targets.
Plasma membrane proteins are highlighted as key targets for new antimicrobial therapies.