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Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
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A Mouse Nasopharyngeal Colonization Model for Group A Streptococcus.

Adrina H J Khemlani1, Thomas Proft2, Jacelyn M S Loh3

  • 1Department of Molecular Medicine and Pathology, School of Medical Sciences and Maurice Wilkins Centre for Biomolecular Discovery, The University of Auckland, Auckland, New Zealand.

Methods in Molecular Biology (Clifton, N.J.)
|May 21, 2020
PubMed
Summary

This study presents a new mouse model for Group A Streptococcus (GAS) colonization in the upper respiratory tract. This model aids in understanding host-bacteria interactions and developing vaccines to reduce GAS carriage.

Keywords:
FVB/n mouseMouse modelNasopharyngeal colonization

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Area of Science:

  • Microbiology
  • Immunology
  • Infectious Diseases

Background:

  • Group A Streptococcus (GAS) is a common human pathogen, frequently colonizing the upper respiratory tract asymptomatically.
  • Symptomatic GAS infections, such as pharyngitis, cause significant morbidity, economic losses, and potential autoimmune complications.
  • Animal models are crucial for studying asymptomatic colonization and evaluating vaccine strategies.

Purpose of the Study:

  • To develop and characterize a mouse model for nasopharyngeal colonization by Group A Streptococcus (GAS).
  • To provide a tool for dissecting host-pathogen interactions during asymptomatic GAS carriage.
  • To facilitate the evaluation of vaccine efficacy in reducing bacterial burden.

Main Methods:

  • Establishment of a mouse model through nasal challenge with GAS.
  • Evaluation of bacterial burden in the nasopharyngeal region of susceptible mice.
  • Characterization of colonization dynamics and host responses.

Main Results:

  • Successful establishment of GAS colonization in the mouse nasopharynx.
  • Quantification of bacterial burden over time post-challenge.
  • Demonstration of the model's utility for studying GAS carriage.

Conclusions:

  • The developed mouse model effectively mimics asymptomatic nasopharyngeal colonization by GAS.
  • This model serves as a valuable platform for investigating host-GAS interactions.
  • It is a promising tool for preclinical assessment of vaccines targeting GAS carriage.