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Glycine metabolomic changes induced by anticancer agents in A549 cells
Kaiqiang Guo1, Yin Cao1, Zan Li1
1Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen, 361102, China.
Amino Acids
|May 21, 2020
Summary
Anticancer drugs alter glycine metabolism in A549 cancer cells, affecting pathways like pyruvate and purine metabolism. Different drugs cause distinct metabolic changes, offering insights for targeted cancer therapies.
Area of Science:
- Metabolomics
- Cancer Biology
- Pharmacology
Background:
- Glycine is crucial for rapidly proliferating cancer cells, making its metabolism a potential therapeutic target.
- The impact of anticancer drugs on glycine metabolism remains largely unexplored.
Purpose of the Study:
- To investigate how anticancer drugs affect glycine metabolism in A549 lung cancer cells.
- To identify specific metabolic alterations induced by various chemotherapy agents.
Main Methods:
- Analysis of 34 glycine metabolites in A549 cells treated with different anticancer drugs.
- Application of Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discrimination Analysis (OPLS-DA) for data interpretation.
Main Results:
- All tested anticancer agents altered glycine metabolism, impacting pyruvate metabolism, betaine aldehyde, and 5'-phosphoribosylglycinamide.
- Cisplatin, camptothecin, and SAHA caused broader metabolite down-regulation compared to afatinib, gefitinib, and targretin.
- Afatinib, gefitinib, and targretin treatments significantly disturbed glycine, serine, threonine, and purine metabolism.
- Cisplatin, camptothecin, and SAHA treatments primarily perturbed glycine, serine, threonine, cysteine, and methionine metabolism.
Conclusions:
- Different anticancer agents induce distinct metabolomic profiles in A549 cells.
- Understanding these drug-induced metabolic changes provides insights for developing novel cancer treatments targeting glycine metabolism.
