Progress on genome-wide CRISPR/Cas9 screening for functional genes and regulatory elements.

Area of Science:

• Genomics
• Molecular Biology
• Biotechnology

Background:

• The CRISPR/Cas9 system is a widely adopted genome editing technology.
• Nuclease-dead Cas9 (CRISPR/dCas9) variants enable functional genomics studies without altering DNA sequences.
• CRISPR/Cas9 is instrumental in understanding disease mechanisms and identifying drug targets.

Purpose of the Study:

• To review research progress in high-throughput CRISPR/Cas9 screening for genome-wide functional gene and regulatory element discovery.
• To highlight the applications of the CRISPR/Cas9 system in animal cell research.

Main Methods:

• High-throughput CRISPR/Cas9 screening.
• Loss-of-function (LOF) and gain-of-function (GOF) screening approaches.
• Non-coding gene scanning using CRISPR/dCas9.

Main Results:

• CRISPR/Cas9 screening effectively identifies functional genes and regulatory elements across the entire genome.
• The system provides valuable insights into complex biological processes.
• Applications extend to animal genetics and breeding.

Conclusions:

• High-throughput CRISPR/Cas9 screening is a powerful approach for functional genomics.
• The CRISPR/Cas9 system offers significant potential for gene editing research and applications in animal science.