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Updated: Dec 21, 2025
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Cysteine/Histidine-Dependent Amidohydrolase/Peptidase (CHAP)-Displayed Nano Phages: Antimicrobial Function against
Golnar Rahimzadeh1, Pooria Gill2, Mohammad Sadegh Rezai1
1Pediatric Infectious Diseases Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Background:
Emergence and prevalence of multi drug resistance strains such as Methicillin-Resistant Staphylococcus aureus (MRSA) call for new antibacterial option. Endolysins as a new option is suggested. The phage display technique is suggested for production of recombinant endolysins. The recombinant endolysins displayed nano phages specifically lysis bacteria, which penetrate to the depth of tissue and the effective dose is reduced.
Methods:
CHAPK gene was ligated in T7Select vector arms in T7Select10-3b cloning kit. To produce recombinant nano phages, ligation reaction was added directly to the packaging extract. Recombinant nano phages were amplified by Double Layer Agar assay (DLA). The recombinant nano phages were characterized using TEM. Size of recombinant nano phages was determined using DLS. The spot test was performed to confirm CHAPk -displayed on the surface of nano phages. The turbidimetry was used to investigate lytic activity of recombinant nano phages against MRSA ATCC No. 33591.
Results:
The results showed recombinant nano phages belonged to order Caudovirales and family Podoviridae with titer 2×107 PFU/ml. According to the results of DLS, size of recombinant nano phages was 71 nm. Formation inhibition zone confirmed the presence of CHAPk on the surface of nano phage phenotypically. The turbidimetry showed lytic activity recombinant nano phages against MRSA after 5 min.
Conclusion:
This study suggests that CHAPk -displayed nano phages can be effective in MRSA infections.
Insights
New nano phages displaying CHAPk show promise for combating Methicillin-Resistant Staphylococcus aureus (MRSA) infections. These engineered bacteriophage endolysins offer a potential new antibacterial strategy against drug-resistant bacteria.
Area of Science:
- Bacteriophage biology
- Molecular biology
- Antimicrobial resistance
Background:
- Rising prevalence of multidrug-resistant bacteria like Methicillin-Resistant Staphylococcus aureus (MRSA) necessitates novel antibacterial approaches.
- Bacteriophage endolysins are proposed as a new therapeutic option.
- Phage display technology offers a method for producing recombinant endolysins.
Purpose of the Study:
- To produce and characterize recombinant nanophages displaying the endolysin CHAPk.
- To evaluate the efficacy of CHAPk-displayed nanophages against MRSA.
Main Methods:
- The CHAPk gene was cloned into a T7Select vector for nanophage display.
- Recombinant nanophages were amplified and characterized using Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS).
- Lytic activity against MRSA was assessed using turbidimetry.
Main Results:
- Recombinant nanophages, identified as belonging to the Podoviridae family, were produced with a titer of 2x10^7 PFU/ml.
- Nanophages measured 71 nm in size, and the presence of CHAPk on their surface was confirmed.
- Turbidimetry demonstrated rapid lytic activity against MRSA within 5 minutes.
Conclusions:
- CHAPk-displayed nanophages demonstrate significant potential as an effective therapeutic agent against MRSA infections.
- This approach offers a promising new avenue for developing antibacterial strategies against resistant pathogens.
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