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A minor structure modification serendipitously leads to a highly carbapenemase-specific fluorogenic probe
Jie Wang1, Weipan Xu, Shuyuan Xue
1State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. xiehexin@ecust.edu.cn.
Organic & Biomolecular Chemistry
|May 21, 2020
Abstract:
Reported herein is a fluorogenic probe for the detection of carbapenemase activity. This reagent features carbapenem as an enzyme recognition motif and a carbon-carbon double bond between carbapenem and the fluorophore, exhibiting high specificity to all carbapenemases, including metallo carbapenemases and serine carbapenemases, over other β-lactamases.

