Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

16.9K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
16.9K
Antibody Structure01:10

Antibody Structure

65.0K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structome-TM: complementing dataset assembly for structural phylogenetics by addressing size-based biases.

Bioinformatics advances·2026
Same author

SGGly: a web server for whole-protein, structure-guided analysis of candidate N-linked glycosylation sites.

Nucleic acids research·2026
Same author

A three generation family with VACTERL association is found to have a rare form of diamond-blackfan anaemia.

European journal of human genetics : EJHG·2026
Same author

Transformers as a substrate for structural biology.

Current opinion in structural biology·2026
Same author

mCSM-metal: A Deep Learning Resource to Predict Effect of Mutations on Metal Ion Binding.

Journal of molecular biology·2026
Same author

Systematic evaluation of computational tools to predict the effects of mutations on protein-ligand binding affinity in the absence of experimental structures.

Briefings in bioinformatics·2026

Related Experiment Video

Updated: Dec 21, 2025

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
13:53

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Published on: December 19, 2020

5.3K

mmCSM-AB: guiding rational antibody engineering through multiple point mutations.

Yoochan Myung1,2, Douglas E V Pires1,2,3, David B Ascher1,2,4

  • 1Computational Biology and Clinical Informatics, Baker Institute, Melbourne, VIC 3004, Australia.

Nucleic Acids Research
|May 21, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces mmCSM-AB, a computational tool for predicting antibody binding affinity changes from multiple mutations. It accurately guides antibody design and optimization for personalized medicine applications.

More Related Videos

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.1K
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

8.1K

Related Experiment Videos

Last Updated: Dec 21, 2025

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
13:53

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Published on: December 19, 2020

5.3K
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.1K
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

8.1K

Area of Science:

  • Biochemistry
  • Computational Biology
  • Immunology

Background:

  • Antibody therapeutics are crucial in personalized medicine.
  • Current antibody engineering relies heavily on experimental methods.
  • Existing computational tools for antibody design lack accuracy and handle single mutations only.

Purpose of the Study:

  • To develop an accurate computational method for analyzing the impact of multiple point mutations on antibody-antigen binding affinity.
  • To provide a tool that aids in the rational design and affinity maturation of antibodies.

Main Methods:

  • Curated a dataset of 242 experimentally determined binding affinity changes from multiple mutations in antibody-target complexes.
  • Utilized graph-based signatures and atomic interaction information for analysis.
  • Developed and validated the mmCSM-AB computational approach.

Main Results:

  • The mmCSM-AB approach accurately predicts the consequence of multi-point mutations on antigen binding affinity.
  • Achieved high performance with Pearson's correlations up to 0.95 in cross-validation and blind tests.
  • Outperformed existing computational tools for antibody design.

Conclusions:

  • mmCSM-AB offers a significant advancement in computational antibody design.
  • The tool can effectively guide antibody affinity maturation processes.
  • mmCSM-AB is available as a web-server to assist researchers in antibody engineering.