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MicroRNA-1324 inhibits cell proliferative ability and invasiveness by targeting MECP2 in gastric cancer

X-Y Zhang1, Y-Y Xu, W-Y Chen

  • 1Department of Gastrointestinal and Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, China. 776726739@qq.com.

European Review for Medical and Pharmacological Sciences
|May 21, 2020
PubMed
Abstract

Insights

MicroRNA-1324 is down-regulated in gastric cancer (GCa), inhibiting MECP2. Lower microRNA-1324 levels promote GCa cell proliferation and invasion, highlighting its role in GCa development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GCa) remains a significant global health challenge.
  • Understanding the molecular mechanisms underlying GCa progression is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the expression of microRNA-1324 in GCa.
  • To elucidate the molecular mechanism of microRNA-1324 in GCa development.
  • To explore microRNA-1324 as a potential diagnostic and therapeutic target for GCa.

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to measure microRNA-1324 and MECP2 expression.
  • Cell counting kit-8 (CCK-8), colony formation, and Transwell assays to assess cell proliferation and invasion.
  • Dual-Luciferase reporter assay, qRT-PCR, and Western Blot to confirm the regulatory interaction between microRNA-1324 and MECP2.

Main Results:

  • MicroRNA-1324 was significantly down-regulated in GCa tissues and cell lines.
  • MECP2 expression was significantly up-regulated in GCa tissues and cell lines.
  • MicroRNA-1324 directly targets and inhibits MECP2 expression.
  • Inhibition of microRNA-1324 enhanced GCa cell proliferation and invasion, accelerated cell cycle, and inhibited apoptosis.
  • Inhibition of MECP2 yielded opposite effects, suggesting a critical role in GCa progression.

Conclusions:

  • MicroRNA-1324 is a tumor suppressor microRNA in gastric cancer.
  • The down-regulation of microRNA-1324 contributes to GCa development by up-regulating MECP2.
  • MicroRNA-1324 may serve as a potential biomarker and therapeutic target for gastric cancer.

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