Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A <i>miR-305-5p</i>/Nerfin-1/Cyp18a1 cascade fine-tunes 20-hydroxyecdysone degradation to time pupariation in <i>Drosophila</i>.

Zoological research·2026

Same author

Genetic contributors to postoperative delirium and their implications for dementia outcomes.

Anesthesiology·2026

Same author

Effect of a dynamic wrist-hand orthosis combined with modified constraint-induced movement therapy on upper limb and hand function in acute and sub-acute stroke patients: a randomized controlled trial.

Topics in stroke rehabilitation·2026

Same author

Premixed Lidocaine With Fospropofol Disodium for Safety and Clinical Evaluation Regarding Paresthesia Upon Fospropofol Disodium Injection: A Preclinical Experimental Study and a Randomized Controlled Trial.

MedComm·2026

Same author

Urbanization-Induced Shifts in Microbial Functional Genes of Wetland Nitrogen Cycling Promote Nitrous Oxide (N<sub>2</sub>O) Emissions.

Microorganisms·2026

Same author

Three new flavonoids and three new stilbenes from Macaranga indica with their cytotoxic activity.

Fitoterapia·2026

Search research articles

Related Experiment Video

Updated: Dec 21, 2025

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

Small molecule proteomics quantifies differences between normal and fibrotic pulmonary extracellular matrices.

Xin-Long Wan^1,2, Zhi-Liang Zhou³, Peng Wang³

¹Platform for Radiation Protection and Emergency Preparedness of Southern Zhejiang, School of Public Health and Management, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.

Chinese Medical Journal

|May 21, 2020

Summary

This summary is machine-generated.

This study identified numerous small-molecular-weight proteins in rat lungs affected by pulmonary fibrosis. Extracellular proteins like serum albumin show potential as biomarkers for this respiratory disease.

More Related Videos

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Published on: July 23, 2015

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

Related Experiment Videos

Last Updated: Dec 21, 2025

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Published on: July 23, 2015

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

Area of Science:

Proteomics
Extracellular Matrix Biology
Respiratory Medicine

Background:

Pulmonary fibrosis involves fibroblast proliferation and extracellular matrix (ECM) accumulation.
The composition of small-molecular-weight proteins in lung ECM, especially in fibrosis, remains largely uncharacterized.

Purpose of the Study:

To identify and characterize small-molecular-weight proteins in normal and paraquat-induced fibrotic rat lungs.
To investigate differentially expressed small-molecular-weight proteins (DESMPs) and their potential as biomarkers for pulmonary fibrosis.

Main Methods:

Rats were induced with paraquat (PQ) to model pulmonary fibrosis.
De-cellularized lung scaffolds were analyzed using high-throughput proteomics.
Differentially expressed small-molecular-weight proteins (DESMPs) were identified and analyzed for their distribution and interactions.

Main Results:

Over 1000 small-molecular-weight proteins were quantified, with significant numbers of up-regulated and down-regulated proteins identified in fibrotic lungs.
DESMPs were primarily involved in cellular and organismal processes, with a higher proportion of up-regulated proteins observed.
Extracellular DESMPs, including serum albumin (Alb), showed significant interactions, suggesting their potential roles.

Conclusions:

High-throughput proteomics successfully identified numerous PQ-induced DESMPs in rat lungs.
The identified DESMPs exhibit diverse molecular functions and biological processes relevant to pulmonary fibrosis.
Extracellular proteins such as Alb, Itgb1, Apoa1, P4hb, and Fgg are potential biomarker candidates for pulmonary fibrosis.