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Flow analysis on microcasting with degassed polydimethylsiloxane micro-channels for cell patterning with cross-linked

Yigang Shen^1,2, Nobuyuki Tanaka¹, Hironori Yamazoe³

¹RIKEN Center for Biosystems Dynamics Research, Osaka, Japan.

|May 21, 2020

Summary

Researchers developed a faster method for patterned cell culturing using microcasting with cross-linked albumin. This technique creates cellular patterns on culture dishes, aiding the study of cell behavior in response to geometric conditions.

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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

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Area of Science:

Biomaterials Science
Cell Biology
Microfluidics

Background:

Patterned cell culturing is crucial for understanding cell behavior influenced by geometry.
Previous methods used agarose gel microstructures fabricated by microcasting with polydimethylsiloxane (PDMS) molds.
The influence of solution viscosity on microcasting was previously unknown.

Purpose of the Study:

To investigate the effect of micro-channel dimensions and solution viscosity on microcasting processes.
To develop a faster method for creating patterned cell culture systems.
To fabricate and test a cross-linked albumin microstructure for cell patterning.

Main Methods:

Experimental investigation of solution flow in PDMS micro-channels.
Numerical simulation of micro-channel flow dynamics.
Microcasting of cross-linked albumin solution to create microstructures on cell culture dishes.
Cultivation of mouse skeletal myoblast cell line C2C12 on fabricated microstructures.

Main Results:

Degassed PDMS molds successfully drove solutions with viscosity below 575 mPa·s.
A simulation model was developed to accurately estimate flow rates in micro-channels.
Cross-linked albumin microstructures were fabricated, exhibiting cellular repellency after dehydration.
Successful patterning of C2C12 cells was observed after 7 days of cultivation.

Conclusions:

Microcasting with cross-linked albumin offers a quicker alternative to agarose gel casting for patterned cell culture.
The developed simulation model aids in optimizing microcasting parameters.
This technique facilitates the study of cell-geometry interactions.