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The isolation of carbon dioxide-requiring (carboxyphillic) type 19 pneumococcus (Streptococcus pneumoniae) from
Laboratory Animals
|October 1, 1978
Abstract:
Type 19 pneumococcus was isolated only in anaerobic cultures from tissue fluids of guinea-pigs suffering from exudative pericarditis and pleuropneumonia. The organism was pathogenic to mice even after 40 subcultures. Further study showed it to be carboxyphilic rather than anaerobic. The necessity of specifying various atmospheric conditions in the diagnostic bacteriological isolation routine is emphasized.
Insights
Type 19 pneumococcus, a bacterium causing animal diseases, was found to require specific atmospheric conditions for isolation. It thrives in carbon dioxide-rich environments, not strictly anaerobic ones, impacting diagnostic methods.
Area of Science:
- Bacteriology
- Microbiology
- Animal Pathology
Background:
- Pneumococcus infections can cause severe conditions like pericarditis and pleuropneumonia in animals.
- Accurate isolation of bacterial pathogens is crucial for diagnosis and treatment.
Purpose of the Study:
- To identify the optimal atmospheric conditions for isolating Type 19 pneumococcus.
- To investigate the growth requirements of this specific pneumococcus strain.
Main Methods:
- Bacterial cultures of Type 19 pneumococcus were performed under anaerobic conditions.
- Subculturing was conducted to assess organism viability and pathogenicity.
- Further experiments were done to determine specific atmospheric requirements.
Main Results:
- Type 19 pneumococcus was successfully isolated from guinea-pig tissue fluids exclusively in anaerobic cultures.
- The organism remained pathogenic to mice after extensive subculturing.
- Subsequent analysis revealed the bacterium to be carboxyphilic (CO2-loving) rather than strictly anaerobic.
Conclusions:
- The isolation of Type 19 pneumococcus is dependent on specific atmospheric conditions, particularly a carbon dioxide-rich environment.
- Diagnostic bacteriology protocols must account for these nuanced atmospheric requirements for accurate pathogen identification.