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Twelve loci form a continuous linkage map for human chromosome 18
P O'Connell1, G M Lathrop, M Leppert
1Howard Hughes Medical Institute, University of Utah Health Sciences Center, Salt Lake City 84132.
Genomics
|November 1, 1988
Summary
Researchers mapped human chromosome 18 using 12 genetic markers, revealing significant differences in genetic distance between males and females. This genetic map aids in studying diseases linked to chromosome 18.
Area of Science:
- Human Genetics
- Genomics
- Molecular Biology
Background:
- Chromosome 18 plays a role in various genetic disorders.
- A comprehensive genetic map is essential for disease gene localization.
Purpose of the Study:
- To construct a primary genetic linkage map for human chromosome 18.
- To identify polymorphic markers for future genetic studies.
Main Methods:
- Utilized 11 DNA markers and 1 serological marker (JK).
- Analyzed linkage data from 59 three-generation families.
- Calculated genetic distances in centimorgans (cM) for males and females.
Main Results:
- Developed a genetic map spanning 97 cM in males and 205 cM in females.
- Observed female genetic distances approximately twice those in males.
- Two markers identified highly polymorphic variable number tandem repeat (VNTR) systems.
Conclusions:
- The constructed map provides efficient linkage analysis for chromosome 18-associated diseases.
- The map serves as a foundation for high-resolution mapping of chromosome 18.
- Significant sex-specific differences in recombination rates were noted.