Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Vitro Fertilization01:24

In Vitro Fertilization

773
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
773

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The opportunities and experiences of interprofessional education for undergraduate nursing students on professional experience placement: An integrative literature review.

Nurse education today·2026
Same author

Conserved shifts in sperm small non-coding RNA profiles during mouse and human aging.

The EMBO journal·2026
Same author

Implementation of the SLOE for MFM applications-insights from its first year in practice.

American journal of obstetrics & gynecology MFM·2025
Same author

Neonatal characteristics of children conceived with in vitro fertilization or intrauterine insemination compared with sibling births from unassisted conceptions.

Fertility and sterility·2025
Same author

Genetic and epigenetic landscape of male infertility.

Trends in genetics : TIG·2025
Same author

Risk of mortality in family members of men seeking fertility assessment.

Fertility and sterility·2025
Same journal

Molecular mechanisms of erectile dysfunction in type 1 and type 2 diabetic rats: a multiomics approach.

Asian journal of andrology·2026
Same journal

The long (GGN)n repeat tract in the androgen receptor gene exerts a negative effect on quantitative sperm parameters.

Asian journal of andrology·2026
Same journal

Exploring potential targets and mechanisms of male reproductive toxicity induced by the emerging PFAS GenX and F-53B via network toxicology, molecular docking, and in vivo validation.

Asian journal of andrology·2026
Same journal

Modifiable key factors and semen quality in men undergoing preconception evaluation: a cross-sectional study.

Asian journal of andrology·2026
Same journal

Somatic cell reprogramming into stem cells: approaches, mechanisms, and therapeutic applications.

Asian journal of andrology·2026
Same journal

A homozygous splicing mutation in CCDC39 caused multiple morphological abnormalities of the flagella in an infertile man with primary ciliary dyskinesia.

Asian journal of andrology·2026
See all related articles

Related Experiment Video

Updated: Dec 20, 2025

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
06:12

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex

Published on: January 9, 2018

13.6K

The hamster egg penetration test may decrease intracytoplasmic sperm injection utilization while maintaining high

Yetunde Ibrahim1, Brett Einerson2, Douglas T Carrell1,3,4

  • 1Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Utah, School of Medicine, Salt Lake City, UT 84132, USA.

Asian Journal of Andrology
|May 22, 2020
PubMed
Summary
This summary is machine-generated.

The hamster egg penetration test (HEPT) effectively predicts conventional fertilization rates, similar to sperm morphology assessment. Utilizing HEPT for males with abnormal morphology can reduce intracytoplasmic sperm injection (ICSI) use and costs.

Keywords:
conventional fertilizationhamster egg penetration testintracytoplasmic sperm injectionsemen analysissperm penetration assay

More Related Videos

Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

1.2K
Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring
06:11

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Published on: May 17, 2024

967

Related Experiment Videos

Last Updated: Dec 20, 2025

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
06:12

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex

Published on: January 9, 2018

13.6K
Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

1.2K
Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring
06:11

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Published on: May 17, 2024

967

Area of Science:

  • Reproductive Medicine
  • Andrology
  • In Vitro Fertilization

Background:

  • Sperm analysis, including morphology and the hamster egg penetration test (HEPT), is crucial for assessing male fertility.
  • Intracytoplasmic sperm injection (ICSI) is often used for cases with poor sperm parameters, but its cost-effectiveness and necessity warrant investigation.

Purpose of the Study:

  • To compare the predictive value of the hamster egg penetration test (HEPT) and sperm morphology for conventional fertilization rates in in vitro fertilization (IVF).
  • To evaluate the cost-effectiveness of using HEPT versus universal ICSI in couples with abnormal sperm morphology.

Main Methods:

  • A cohort study stratified IVF cycles based on HEPT and sperm morphology results (normal/abnormal).
  • Calculated mean conventional and intracytoplasmic sperm injection (ICSI) fertilization rates for each group.
  • Performed univariate analysis and a cost-effectiveness analysis comparing HEPT policy to universal ICSI for abnormal morphology.

Main Results:

  • No significant difference in conventional fertilization rates was observed between normal and abnormal morphology groups when HEPT was normal.
  • No difference in fertilization rates between subjects with normal morphology without HEPT and those with normal HEPT without morphology assessment.
  • In simulations with abnormal morphology, a HEPT policy was cost-saving compared to universal ICSI, yielding similar fertilization rates.

Conclusions:

  • The HEPT demonstrates comparable predictive ability to World Health Organization (WHO) 5th edition morphology for successful conventional fertilization.
  • Employing HEPT for males with abnormal morphology can guide the selection of fertilization methods, potentially decreasing ICSI utilization and associated costs.