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Updated: Oct 9, 2026
Murine Model of Intestinal Ischemia-reperfusion Injury
Published on: May 11, 2016
The protective effects of adalimumab on intestinal injury induced with infrarenal aortic occlusion
Ahmet Pergel1, Levent Tümkaya2, Gökhan Demiral1
1Department of General Surgery, Recep Tayyip Erdoğan University Faculty of Medicine, Rize-Turkey.
Background:
The present study aims to observe the effects of ischemia-reperfusion (IR) on small intestines at a molecular level and to prospectively assess the potential preventive role of adalimumab (ADA) and antioxidants.
Methods:
A total of 24 male Sprague-Dawley rats were randomly divided into three groups-a control group, an IR group and an IR+ADA group.
Results:
Although there was no change in SOD levels in the small bowel tissue of the IR group, we observed increased malondialdehyde (MDA) levels and increased numerical density of caspase-3 and TNF-α positive enterocytes p=0.00 and p=0.00, respectively). We also observed that IR caused the degeneration of villus crypt structures.
Conclusion:
We found that ADA treatment reduced MDA levels and decreased the numerical density of caspase-3 and TNF-α positive enterocytes compared to the IR group (p=0.00; p=0.011; p=0.00, respectively). We conclude that ADA can be beneficial in preventing intestinal injury that arises from IR.
