Biomimetic Composite Scaffold Based on Naturally Derived Biomaterials

Area of Science:

• Biomaterials Science
• Biomedical Engineering
• Materials Science

Background:

• Bone defects and regeneration require advanced biomaterials.
• Naturally derived materials offer biocompatible and sustainable alternatives for bone tissue engineering.

Purpose of the Study:

• To develop a novel biomimetic composite scaffold for bone regeneration.
• To utilize eggshell membrane as a bio-template for hydroxyapatite synthesis.
• To enhance the bone regeneration capacity using natural components.

Main Methods:

• Microwave synthesis of biomimetic hydroxyapatite (HAp) using eggshell membrane (ESM) as a bio-template.
• Incorporation of tricalcium phosphate (BA), Gelatin (Gel), and Chitosan (CS) to form the composite scaffold.
• Characterization using spectroscopy and morphology studies, followed by in vitro cytotoxicity assays on amniotic fluid stem cells (AFSC).

Main Results:

• The synthesized HAp (ESM) contained hydroxyapatite and monetite phases.
• The composite scaffold exhibited a porous structure, crucial for biocompatibility.
• In vitro studies confirmed the effective biocompatibility and non-toxicity of the scaffold.

Conclusions:

• An original biocomposite scaffold was successfully developed from naturally derived materials.
• The scaffold demonstrates potential for stimulating cellular response and accelerating bone healing.
• The developed material is biocompatible and synthesized in a non-toxic manner.