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Preparative-scale high-performance liquid chromatography of omega-3 polyunsaturated fatty acid esters derived from
J M Beebe1, P R Brown, J G Turcotte
1Department of Chemistry, University of Rhode Island, Kingston 02881.
Journal of Chromatography
|December 28, 1988
Abstract:
Marine triglyceride-derived omega-3 polyunsaturated fatty acid ethyl esters were separated by preparative high-performance liquid chromatography on a 25-microns octadecyl stationary phase using a ternary isocratic mobile phase of acetonitrile-tetrahydrofuran-water (466:233:300, v/v/v). The highest purity first-run fractions obtained were ethyl esters of the major marine polyunsaturates eicosapentaenoic acid (20:5 omega 3, 97.7%) and docosahexaenoic acid (22:6 omega 3, 93.7%), and the minor polyunsaturate octadecatetraenoic acid (18:4 omega 3, 98.1%).