Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Discrimination between cholesterol and sitosterol for absorption in rats.

I Ikeda1, K Tanaka, M Sugano

  • 1Department of Biochemistry, George Washington University, School of Medicine, Washington, DC 20037.

Journal of Lipid Research
|December 1, 1988
PubMed
Summary

Rats absorb significantly more cholesterol than sitosterol, with 18.2% cholesterol absorbed versus 0.42% sitosterol. This difference persists regardless of dosage or physical state, highlighting distinct intestinal sterol absorption mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparisons of the Effects of Dietary Fats on Serum and Liver Lipid Levels of Rats.

Bioscience, biotechnology, and biochemistry·2016
Same author

Participation of nitric oxide and peroxynitrite in the development of myocardial tissue damage in acute myocardial infarction.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
Same author

Microsatellite instability occurs infrequently in sporadic renal cell carcinoma.

Oncology reports·2011
Same author

Alkaline Trypan Blue as a stain for superficial fungi.

The British journal of dermatology·2008
Same author

Education and imaging. Gastrointestinal: diphyllobothriasis.

Journal of gastroenterology and hepatology·2007
Same author

High-frequency signals (> 400 hz): a new window in electrophysiological analysis of the somatosensory system.

Clinical EEG and neuroscience·2005

Area of Science:

  • Gastroenterology
  • Lipid Metabolism
  • Molecular Biology

Background:

  • Cholesterol and sitosterol are plant and animal sterols with similar structures.
  • Understanding their differential absorption is crucial for managing cholesterol levels and developing phytosterol-based therapies.

Purpose of the Study:

  • To compare the intestinal absorption of cholesterol and sitosterol in rats.
  • To elucidate the mechanisms underlying the differential lymphatic transport of these sterols.

Main Methods:

  • Rats were administered emulsified lipid meals containing radiolabeled cholesterol or sitosterol.
  • Lymphatic absorption, esterification, and association with chylomicron fractions were analyzed.
  • Brush border membrane uptake was assessed in vivo and in vitro.

Related Experiment Videos

Main Results:

  • Lymphatic absorption of cholesterol (18.2%) was significantly higher than sitosterol (0.42%).
  • Cholesterol was predominantly esterified in lymph, while sitosterol remained largely free.
  • Sitosterol showed lower association with mucosal tissue and brush border membranes compared to cholesterol.

Conclusions:

  • Rats exhibit a marked preference for absorbing cholesterol over sitosterol via the lymphatic system.
  • Differential esterification and intracellular trafficking contribute to the reduced lymphatic recovery of sitosterol.
  • These findings provide insights into the molecular basis of sterol absorption and potential targets for lipid-lowering strategies.