Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chromatin- and actin-mediated mitochondrial streaming leads to patterning of mitochondrial distribution in oocytes.

Nature communications·2026

Same author

Microfluidic-assisted self-assembly of information-bearing oligomers.

Nanoscale·2026

Same author

Retinal Blood Flow Decreases After Treatment with Laser Photocoagulation for Retinopathy of Prematurity.

Ophthalmology science·2026

Same author

Accessibility and readability of Spanish and Portuguese-translated online information about common eye conditions: A cross-sectional content analysis study.

Journal of the National Medical Association·2026

Same author

Refined trajectory smoothing and deep learning classification of human sperm motility.

Human reproduction (Oxford, England)·2026

Same author

Functional vs anatomical cortico-striatal connectivity in the macaque brain.

Translational psychiatry·2025

Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026

Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026

Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026

Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026

Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026

Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Paper-Based Acoustofluidics for Separating Particles and Cells.

Jason Zhou¹, Ruhollah Habibi¹, Farzan Akbaridoust¹

¹Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia.

Analytical Chemistry

|May 23, 2020

Summary

This summary is machine-generated.

This study introduces a novel paper-based acoustic method to precisely control microparticle and cell movement. This technology enables efficient size-dependent separation and concentration for advanced diagnostics.

More Related Videos

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Published on: March 6, 2016

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Published on: January 21, 2021

Related Experiment Videos

Last Updated: Jun 27, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Published on: March 6, 2016

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Published on: January 21, 2021

Area of Science:

Microfluidics
Acoustic manipulation
Biotechnology

Background:

Paper-based microfluidics offers a versatile platform for fluid handling in diagnostics and chemistry.
Controlling analyte transport for concentration and selection in paper remains a significant challenge.

Purpose of the Study:

To develop a rapid and effective method for size-dependent control of microparticle and cell movement within paper-based devices.
To demonstrate the capability of acoustic manipulation for trapping, concentrating, and releasing particles and cells.

Main Methods:

Integration of paper-based microfluidics with surface acoustic waves (SAW).
Utilizing SAW to manipulate the movement of microparticles and cells based on their size.
Assessing collection efficiency and size-dependent separation capabilities.

Main Results:

Achieved over 98% collection efficiency for microparticles using the paper-based SAW approach.
Demonstrated size-dependent isolation of microparticles (1.1, 3.2, and 5 μm).
Successfully trapped and concentrated human prostate cancer PC3 cells at a specific site.

Conclusions:

The paper-based SAW approach provides a powerful tool for selective manipulation of microparticles and cells.
This method enables the development of low-cost, high-performance analytical tools for complex samples.
Potential applications in medical diagnostics and analytical chemistry are significant.