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Updated: Dec 20, 2025
08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
8.3K
Notch Regulates Fibrocartilage Stem Cell Fate and Is Upregulated in Inflammatory TMJ Arthritis
A Ruscitto1, V Scarpa1, M Morel1
1Cartilage Biology and Regenerative Medicine Laboratory, College of Dental Medicine, Columbia University Irving Medical Center, New York, NY, USA.
Journal of Dental Research
|May 23, 2020
Summary
The Notch pathway is crucial for temporomandibular joint (TMJ) development and homeostasis. Inhibiting Notch may offer a new therapeutic strategy for TMJ osteoarthritis.
Area of Science:
- Biochemistry
- Developmental Biology
- Orthopedics
Background:
- The Notch pathway regulates cartilage development and plays a dual role in adult cartilage health and osteoarthritis.
- The temporomandibular joint (TMJ) is susceptible to injury and osteoarthritis, yet the role of Notch signaling in TMJ is poorly understood.
Purpose of the Study:
- To investigate the role of Notch signaling in TMJ development and fibrocartilage stem cell (FCSC) fate.
- To explore Notch's potential as a therapeutic target for TMJ osteoarthritis.
Main Methods:
- Utilized a Notch reporter mouse to track Notch activity in TMJ stem cells.
- Employed a TNF-α-induced TMJ inflammatory arthritis mouse model.
- Assessed FCSC differentiation and gene expression following Notch pathway manipulation.
Main Results:
- FCSCs in the TMJ superficial niche exhibit Notch activity during development.
- Constitutive Notch activation promoted chondrogenic and osteogenic differentiation while inhibiting adipogenesis in FCSCs.
- Notch signaling components were upregulated in an inflammatory TMJ arthritis model, correlating with cartilage-to-bone transdifferentiation.
- Global Notch inhibition reduced FCSC osteogenic and chondrogenic differentiation.
Conclusions:
- Notch signaling is essential for TMJ development, FCSC fate specification, and maintaining joint homeostasis.
- Targeting the Notch pathway presents a potential therapeutic avenue for TMJ osteoarthritis.
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