Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cells of the Epidermis01:24

Cells of the Epidermis

6.5K
The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
6.5K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.9K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.9K
Layers of the Epidermis01:21

Layers of the Epidermis

7.4K
The epidermis, the outermost layer of the skin, is composed of several distinct layers. From deep to superficial, the layers of the epidermis are as follows:
Stratum Basale
Stratum basale, also known as the stratum germinativum, is the deepest layer of the epidermis. It is composed of a single layer of actively dividing cells called basal cells or basal keratinocytes. These cells constantly undergo cell division to replenish the upper layers of the epidermis. Additionally, melanocytes, which...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spark plasma sintered titania-graphene oxide: A toothless keratoprosthesis for end-stage corneal blindness.

Biomaterials·2026
Same author

The Role of Epigenetics in Corneal Fibrosis.

Epigenomes·2026
Same author

Spatial patterning of biomimetic signals in corneal regeneration.

Advanced drug delivery reviews·2026
Same author

Ex Vivo Assessment of Excimer Laser-Assisted Lenticule Customization.

Translational vision science & technology·2026
Same author

Human Corneal Stromal Stem Cell Treatment Reduces Established Opacities in Chronic Corneal Scarring.

Cells·2026
Same author

Exposure of human corneal epithelial cells to microplastic particles induces a phase-specific cytokine response.

Environmental pollution (Barking, Essex : 1987)·2026
Same journal

Corneal deformation mapping and FE-based strain analysis via digital image correlation: Biomechanical changes after CXL and laser refractive surgery.

Experimental eye research·2026
Same journal

Tanshinone IIA inhibits choroidal neovascularization and restores outer blood-retinal barrier function in Vldlr knockout mice.

Experimental eye research·2026
Same journal

Understanding the ocular accumulation of mefuparib and its N-dealkylation metabolite: Pharmacokinetics, melanin affinity, and cellular disposition.

Experimental eye research·2026
Same journal

Mitochondrial Dysfunction and Diabetic Retinopathy: Research Progress from Pathogenic Mechanisms to Therapeutic Targets.

Experimental eye research·2026
Same journal

Middle-wavelength green ambient light attenuates lens-induced myopia progression and is associated with suppression of the Wnt/β-catenin signaling pathway in guinea pigs.

Experimental eye research·2026
Same journal

Experimental Corneal Alkali Burn Models: Methodological Standards, Biological Outcomes, and Translational Gaps.

Experimental eye research·2026
See all related articles

Related Experiment Video

Updated: Dec 20, 2025

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

17.2K

Keratocyte biology.

Gary H F Yam1, Andri K Riau2, Martha L Funderburgh1

  • 1Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.

Experimental Eye Research
|May 23, 2020
PubMed
Summary
This summary is machine-generated.

Corneal keratocytes are vital for maintaining clear vision. Regenerating these cells using ex vivo propagation shows promise for restoring corneal transparency in animal models.

Keywords:
CorneaCorneal diseasesCorneal managementStromal cell therapyStromal keratocytes

More Related Videos

Generating Primary Cultures for Keratinocyte Live Cell Imaging
07:38

Generating Primary Cultures for Keratinocyte Live Cell Imaging

Published on: May 2, 2025

424
Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
10:51

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

32.6K

Related Experiment Videos

Last Updated: Dec 20, 2025

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

17.2K
Generating Primary Cultures for Keratinocyte Live Cell Imaging
07:38

Generating Primary Cultures for Keratinocyte Live Cell Imaging

Published on: May 2, 2025

424
Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
10:51

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

32.6K

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Corneal keratocytes are crucial for maintaining corneal transparency and homeostasis.
  • Disruption of keratocyte function by trauma leads to corneal haze and vision loss.
  • Understanding stromal cell behavior is key for treating corneal pathologies.

Purpose of the Study:

  • To investigate the biology and behavior of corneal keratocytes and associated stromal cells.
  • To explore the potential of ex vivo propagated keratocytes for corneal regeneration.
  • To evaluate translational applications of stromal cell therapies.

Main Methods:

  • Ex vivo propagation of keratocytes and stromal stem cells.
  • Animal models of corneal haze and other disorders.
  • Stromal injection and incorporation into bioscaffolds for therapeutic delivery.

Main Results:

  • Ex vivo propagated keratocytes and stromal stem cells can be used for therapeutic applications.
  • Restoration of corneal transparency and native stromal tissue regeneration observed in animal models.
  • Successful application in models of corneal haze and other disorders.

Conclusions:

  • Ex vivo expansion and transplantation of corneal keratocytes and stromal stem cells offer a promising therapeutic strategy.
  • This approach can restore corneal transparency and regenerate stromal tissue.
  • Potential applications include treating corneal haze and other vision-impairing conditions.