Simultaneous biodegradation of methane and styrene in biofilters packed with inorganic supports: Experimental and...

Four upflow 0.018 m³ biofilters (3 beds), B-ME, B-200, B-500 and B-700, all packed with inorganic materials, were operated at a constant air flow rate of 0.18 m³ h^-1 to eliminate methane (CH₄), a harmful greenhouse gas (GHG), and styrene (C₈H₈), a carcinogenic volatile organic compound (VOC). The biofilters were irrigated with 0.001 m³ of recycled nutrient solution (NS) every day (flow rate of 60 × 10^-3 m³ h^-1). Styrene inlet load (IL) was kept constant in each biofilter. Different CH₄-ILs varying in the range of 7-60 gCH₄ m^-3 h^-1 were examined in B-ME (IL of 0 gC₈H₈ m^-3 h^-1), B-200 (IL of 9 gC₈H₈ m^-3 h^-1), B-500 (IL of 22 gC₈H₈ m^-3 h^-1) and B-700 (IL of 32 gC₈H₈ m^-3 h^-1). Finally, the effect of C₈H₈ on the macrokinetic parameters of CH₄ biofiltration was studied based on the Michaelis-Menten model. Average C₈H₈ removal efficiencies (RE) varying between 64 and 100% were obtained at CH₄-ILs increasing from 7 to 60 gCH₄ m^-3 h^-1 and for C₈H₈-ILs range of 0-32 gC₈H₈ m^-3 h^-1. More than 90% of C₈H₈ was removed in the bottom and middle beds of the biofilters. By increasing C₈H₈-IL from 0 to 32 gC₈H₈ m^-3 h^-1, maximal EC in Michaelis-Menten model and macrokinetic saturation constant declined from 311 to 39 g m^-3 h^-1 and from 19 to 2.3 g m^-3, respectively, which confirmed that an uncompetitive inhibition occurred during CH₄ biofiltration in the presence of C₈H₈.