Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long Distance Non-Contact Dermoscopy: Technological Foundations, Clinical Applications, and Future Directions.

International journal of dermatology·2026
Same author

Functionalized Quantum Dot-Based Portable Sensor for Dual Detection of Stress Biomarkers in Blood Serum and Saliva.

Journal of biophotonics·2026
Same author

Four-modal device comprising optical coherence tomography, photoacoustic tomography, ultrasound, and Raman spectroscopy developed for in vivo skin lesion assessment.

Biomedical optics express·2026
Same author

Polarimetric analysis of the Alzheimer's pathology in excised mouse brain tissue.

Journal of biomedical optics·2026
Same author

Correction: One-pot aqueous synthesis of highly strained CdTe/CdS/ZnS nanocrystals and their interactions with cells.

RSC advances·2025
Same author

High-resolution imaging system for integration into intelligent noncontact total body scanner.

Journal of biomedical optics·2025

Related Experiment Video

Updated: Dec 20, 2025

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

13.3K

SPR Biosensor Based on Polymer Multi-Mode Optical Waveguide and Nanoparticle Signal Enhancement.

Johanna-Gabriela Walter1, Alina Eilers1, Lourdes Shanika Malindi Alwis2

  • 1Institute of Technical Chemistry, Leibniz University of Hannover, 30167 Hannover, Germany.

Sensors (Basel, Switzerland)
|May 24, 2020
PubMed
Summary

A novel surface plasmon resonance (SPR) biosensor uses a multi-mode polymer waveguide for sensitive biomolecule detection. This cost-effective lab-on-a-chip device shows potential for rapid, multiplexed biomarker analysis.

Keywords:
aptamerbiosensorgold-nanoparticlelab-on-a-chipmulti-modeplanar-opticalsensorsurface plasmon resonance (SPR)waveguide

More Related Videos

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.6K
Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

10.6K

Related Experiment Videos

Last Updated: Dec 20, 2025

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

13.3K
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.6K
Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

10.6K

Area of Science:

  • Biomedical Engineering
  • Optoelectronics
  • Nanotechnology

Background:

  • Surface plasmon resonance (SPR) is a label-free optical technique widely used for detecting molecular interactions.
  • Existing SPR biosensors often require complex instrumentation and are costly, limiting their widespread application.
  • There is a need for sensitive, low-cost, and portable biosensing platforms for point-of-care diagnostics.

Purpose of the Study:

  • To develop a novel SPR biosensor utilizing a planar-optical multi-mode (MM) polymer waveguide.
  • To demonstrate the sensor's capability for detecting biomolecules in the nano-molar (nM) range.
  • To evaluate the potential for low-cost, disposable lab-on-a-chip applications and multiplexed detection.

Main Methods:

  • Fabrication of a planar-optical multi-mode polymer waveguide structure for SPR sensing.
  • Characterization of the basic sensor's sensitivity and resolution using refractive index changes.
  • Integration of an aptamer-functionalized, gold-nanoparticle (AuNP)-enhanced sandwich assay for specific biomarker detection (C-reactive protein - CRP).

Main Results:

  • The basic SPR sensor exhibited a sensitivity of 608.6 nm/RIU and a measurement resolution of 4.3 × 10-3 RIU.
  • The enhanced SPR biosensor successfully detected C-reactive protein (CRP) with a response of 0.118 nm/nM.
  • The multi-mode polymer waveguide design enables a simple and cost-effective platform.

Conclusions:

  • The developed SPR biosensor based on a multi-mode polymer waveguide offers high sensitivity and resolution for biomolecule detection.
  • The aptamer-functionalized, AuNP-enhanced assay provides specific and sensitive detection of biomarkers like CRP.
  • The sensor is suitable for low-cost, disposable lab-on-a-chip applications, with potential for fast and multiplexed biomarker analysis on a single platform.